HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent; one of the twenty Good's buffers. Offer ends Nov. 30th, 2020. Compared with other buffers such as PBS (phosphate buffered saline) and TRIS, HEPES has higher stability in maintaining the pH values of the cell culture media[3][4][5], that’s also the reason why HEPES is widely used in cell culture , tissue culture, protein purification and extraction, immunoprecipitation, cell lysis, live cell imaging and other biological/biochemical researches. Sodium bicarbonate is nutritionally necessary for most cells, so HEPES should be added in addition to, not in place of, sodium bicarbonate. Copyright 2020 MP Biomedicals. HEPES powder is resistant to high temperatures with its melting point reaching 200℃, thus, it will not get degraded by autoclaving. Box prices only valid with purchase of full box. Sign up today to receive important product updates and exclusive promotions. HEPES has no nutritional benefit to cells. For Research Use Only. Check out the uses, concerns, useful tips and references related to HEPES buffer in varied applications, such as electrophoresis, cell culture and chromatography. 99.5 %. Corning® HEPES IMPORTANT UPDATE: Online ordering is now available for Media and Biologics products and select cell culture vessels. https://www.intechopen.com/books/biomedical-tissue-culture/culture-conditions-and-types-of-growth-media-for-mammalian-cells, https://www.microbiologyresearch.org/docserver/fulltext/jgv/2/2/JV0020020309.pdf?expires=1569478206&id=id&accname=guest&checksum=E838F092CC37D8C426A562FACC3BA003, The formula in the Pharmaceutical field-Pharma Grade Trometamol, The 4 basic classifications of Cryopreservation Procedure you must be know. © 1994-2019 Corning Incorporated All Rights Reserved. For the best experience on our site, be sure to turn on Javascript in your browser. HEPES has been used in a wide variety of applications, including tissue culture. Applications of HEPES buffer. When cultivated in stoppered flasks, cells grew equally well or even better in TES- and HEPES-buffered medium than in NaHCO 3-buffered medium irrespective of cell culture density. It is therefore strongly advised to keep HEPES-containing solutions in darkness as much as possible to prevent oxidation. ): min. HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent; one of the twenty Good's buffers. It has no nutritional benefit to cells. Need technical support? HEPES is frequently added to cell culture media in applications when pH maintenance is critical and normal bicarbonate buffering is not sufficient. However, there are so many biological buffers and alternatives available for cell culture on the market, why HEPES? Co., Ltd - Fine Chemicals Division. It is commonly used to buffer cell … The most commonly used concentration is 25mM. All rights reserved. ): min. Sie haben kein Konto? Hepes . In GIBCO DMEM it is at a concentration of 25mM. Search Media Formulation Tool Find the right Gibco media formulation for DMEM, DMEM/F-12, MEM, and RPMI-1640 media. Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. [1] Lepe-Zuniga et al. HEPES is like water in that its dissociation decreases as the temperature decreases. The level of HEPES in cell culture media may vary from 10mM to 25mM. For more information, please contact our customer service department. al., Biochemistry 1966). Addition of HEPES provides supplemental buffering to cell culture medium at pH 7.2 through 7.6. Thermo Fisher Scientific. Cell Culture & Transfection Learning Center Access cell culture and transfection educational resources for better experiment planning and execution. Subscribe to our mail list and be the first to receive exclusive content from Hopax in your mailbox: Be the first to know about Hopax's special offers, new blog posts and relevant news about our industry. Gibco HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent and is categorized as a "Good" buffer which derives from a set of buffers described by Dr. Norman Good and his colleagues in 1966 (Good et. In GIBCO DMEM/F-12 it is at a 15mM. HEPES has been described as one of the best all-purpose buffers available for biological research. The most commonly used buffering system for media is bicarbonate. It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES buffer does not confer cytotoxic effects on cells and thus can be used in animal cell cultures. IMPORTANT UPDATE:  Online ordering is now available for Media and Biologics products and select cell culture vessels.For assistance with other products, please  click here. Please contact us for the high-quality HEPES!! "Good buffers" such as HEPES are attributed with the following characteristics: HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media. Usage Statement . SDS; TDS; COA; Recommended prices only. HEPES for cell culture Assay (titr. This is due to its nutritional benefits despite the reduced buffering capacity at physiological pH. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2.